Replication of measles virus in human lymphocytes.

Replication of measles virus in human lymphocytes

Replication of measles virus was restricted in human peripheral blood mononuclear cells (PBMC). However, in in vitro-infected, unstimulated cells, active synthesis of viral RNA and proteins occurred, while the release of infectious virus could not be detected. Stimulation with PHA caused a productive infection cycle comparable to the lytic infection. Replication of viral RNA was demonstrated in...

Role of Interferon in Replication of Virulent and Attenuated Strains of Measles Virus

Activation of measles virus from silently infected human lymphocytes

Lymphocytes were incubated with measles virus for 4 days in the absence of a lymphocyte stimulating agent. Such nonstimulated lymphocytes, infected with measles virus, did not express the virus antigens that are detectable by cytotoxic antibodies. Approximately 1 out of 5,000, or even fewer, of such lymphocytes produced virus as demonstrated by the infectious center assay; in the supernate only...

Electron microscopy of measles virus replication.

Replication of measles virus in HeLa cells was examined by electron microscopy with ultrathin sectioning and phosphotungstic acid negative staining methods. The cytoplasmic inclusion bodies consisted of masses of helical nucleocapsid which was similar in structure to the nucleocapsid found in measles virions. The cytoplasmic helical nucleocapsid appeared to align near the HeLa cell membrane, an...

Sphingosine kinase 1 regulates measles virus replication.

Measles virus (MV) manipulates host factors to facilitate virus replication. Sphingosine kinase (SK) is an enzyme catalyzing the formation of sphingosine 1-phosphate and modulates multiple cellular processes including the host defense system. Here, we determined the role of SK1 in MV replication. Overexpression of SK1 enhanced MV replication. In contrast, inhibition of SK impaired viral protein...